Hotspot

trnC-GCA-petN

LSC · 28293-29122 · 830 bp

Back to Streptocarpus

Pi 0.00402

MarkerSeek score 60.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 8
pi: 0.00402
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	904-940 bp	1.0	83.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	902-938 bp	1.0	83.1
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	956-992 bp	1.0	82.5
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	954-990 bp	1.0	82.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	905-941 bp	1.0	82.5