Hotspot

psbK-psbI

LSC · 8006-8479 · 474 bp

Back to Streptocarpus

Pi 0.00436

MarkerSeek score 56.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 6
pi: 0.00436
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	591-634 bp	1.0	76.9
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	586-629 bp	1.0	76.9
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	TCTTAGTCTTCACGTCCAGGA	45.0	47.6	58.1	57.8	597-640 bp	1.0	75.1
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TCTTAGTCTTCACGTCCAGGA	50.0	47.6	57.9	57.8	592-635 bp	1.0	75.1
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	583-626 bp	1.0	74.9