Hotspot

ndhG-ndhI

SSC · 123766-124152 · 387 bp

Back to Stranvaesia

Pi 0.0103

MarkerSeek score 62.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 387
variable_sites: 6
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.0	56.4	497-503 bp	1.0	69.3
ndhG-ndhI_p2	AATACCACCCCCATACCTCC	TCAAATTGCTTTGGGTCGTT	55.0	40.0	58.2	56.4	552-558 bp	1.0	67.9
ndhG-ndhI_p3	TGTATTGGTCCGGGCAAATC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.2	56.4	492-498 bp	1.0	67.5
ndhG-ndhI_p4	AATCATGTATTGGTCCGGGC	ATCAAATTGCTTTGGGTCGT	50.0	40.0	58.0	56.2	498-504 bp	1.0	67.5
ndhG-ndhI_p5	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTTT	50.0	38.1	58.0	57.2	497-503 bp	1.0	67.3