Hotspot

rpl20-rps12

LSC · 72508-73276 · 769 bp

Back to Stranvaesia

Pi 0.00087
MarkerSeek score 60.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
769
variable_sites
1
pi
0.00087
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 919-925 bp 1.0 75.4
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 918-924 bp 1.0 75.4
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA CAGCGACGAGGAACATGTAC 50.0 55.0 57.9 58.7 882-888 bp 1.0 73.9
rpl20-rps12_p4 TTGAATGAGCCCCTCGAAAG CAGCGACGAGGAACATGTAC 50.0 55.0 57.9 58.7 881-887 bp 1.0 73.9
rpl20-rps12_p5 TTGAATGAGCCCCTCGAAAG AGCGACGAGGAACATGTACT 50.0 50.0 57.9 58.8 880-886 bp 1.0 73.1