Hotspot

trnF-GAA-ndhJ

LSC · 51695-52449 · 755 bp

Back to Stranvaesia

Pi 0.000897

MarkerSeek score 64.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 1
pi: 0.000897
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	892-945 bp	1.0	83.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	856-909 bp	1.0	83.0
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	880-933 bp	1.0	82.3
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	905-958 bp	1.0	82.2
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	916-969 bp	1.0	82.1