Hotspot

rps16-trnQ-UUG

LSC · 6378-7168 · 791 bp

Back to Stranvaesia

Pi 0.00169

MarkerSeek score 60.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	875-895 bp	1.0	78.3
rps16-trnQ-UUG_p2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	885-905 bp	1.0	76.6
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	852-872 bp	1.0	76.6
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	886-906 bp	1.0	76.5
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	937-957 bp	1.0	76.1