Hotspot

rpl32-trnL-UAG

SSC · 105924-106415 · 492 bp

Back to Stipagrostis

Pi 0.0203

MarkerSeek score 60.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 492
variable_sites: 15
pi: 0.0203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGTGGCAACGAGTATCCAAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.1	57.9	600-615 bp	1.0	74.3
rpl32-trnL-UAG_p2	AGTGGCAACGAGTATCCAAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.1	57.8	593-608 bp	1.0	73.9
rpl32-trnL-UAG_p3	AGTGGCAACGAGTATCCAAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.1	58.2	614-629 bp	1.0	73.8
rpl32-trnL-UAG_p4	GGCAACGAGTATCCAAAACC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.1	57.9	597-612 bp	1.0	73.8
rpl32-trnL-UAG_p5	AGTGGCAACGAGTATCCAAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.1	57.8	598-613 bp	1.0	73.7