Hotspot

atpA-trnR-UCU

LSC · 36009-36171 · 163 bp

Back to Stipagrostis

Pi 0.0123

MarkerSeek score 39.10

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 3
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TGAACGGTTTTCCCTTCAGG	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	216-225 bp	1.0	67.4
atpA-trnR-UCU_p2	TAGCAAGACATTCACCGAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.0	57.3	276-285 bp	1.0	67.2
atpA-trnR-UCU_p3	AGGAACAGCTTGAACGGTTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	226-235 bp	1.0	67.1
atpA-trnR-UCU_p4	AGCTTGAACGGTTTTCCCTT	GGTTCAAATCCTATTGGACGC	45.0	47.6	57.9	57.3	220-229 bp	1.0	67.0
atpA-trnR-UCU_p5	GAACGGTTTTCCCTTCAGGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	215-224 bp	1.0	65.4