Hotspot

trnG-GCC-trnfM-CAU

LSC · 12228-12593 · 366 bp

Back to Stipagrostis

Pi 0.0164

MarkerSeek score 64.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 9
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTGTAGCGAGCGTAGTTCAA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.0	57.8	489-496 bp	1.0	79.4
trnG-GCC-trnfM-CAU_p2	GAAGGTACGGGTTCGATTCC	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.1	57.8	444-451 bp	1.0	79.2
trnG-GCC-trnfM-CAU_p3	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	484-491 bp	1.0	79.0
trnG-GCC-trnfM-CAU_p4	GTGTAGCGAGCGTAGTTCAA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.0	58.3	504-511 bp	1.0	78.9
trnG-GCC-trnfM-CAU_p5	GAAGGTACGGGTTCGATTCC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	58.1	58.3	459-466 bp	1.0	78.7