Hotspot

rpl32-trnL-UAG

SSC · 106288-107022 · 735 bp

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Pi 0.00695
MarkerSeek score 55.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
735
variable_sites
56
pi
0.00695
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 815-853 bp 1.0 86.9
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 814-852 bp 1.0 86.9
rpl32-trnL-UAG_p3 AGGGTTTTTCTGGGCAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 817-855 bp 1.0 86.5
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 818-856 bp 1.0 86.4
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 787-825 bp 1.0 86.2