Hotspot

petA-psbJ

LSC · 61382-62196 · 815 bp

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Pi 0.00489
MarkerSeek score 59.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
815
variable_sites
39
pi
0.00489
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC GCTGGTATTCCTGTGATTGGT 50.0 47.6 57.9 58.0 879-1000 bp 1.0 78.8
petA-psbJ_p2 TGTTCTTCTTCGCATCCGTT GCTGGTATTCCTGTGATTGGT 45.0 47.6 57.8 58.0 872-993 bp 1.0 77.9
petA-psbJ_p3 TTGTTCTTCTTCGCATCCGT GCTGGTATTCCTGTGATTGGT 45.0 47.6 57.8 58.0 873-994 bp 1.0 77.9
petA-psbJ_p4 GGCCTTTTGTTCTTCTTCGC AGCTGGTATTCCTGTGATTGG 50.0 47.6 57.9 57.7 880-1001 bp 1.0 76.5
petA-psbJ_p5 GGCCTTTTGTTCTTCTTCGC TGGCTGATAGGTACTGTAGCT 50.0 47.6 57.9 57.7 897-1018 bp 1.0 76.2