Hotspot

ycf4-cemA

LSC · 59041-59493 · 453 bp

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Pi 0.00301
MarkerSeek score 50.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
453
variable_sites
12
pi
0.00301
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 ACAAAAAGCTGCCGAATTGG CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.8 58.2 512-575 bp 0.96 81.0
ycf4-cemA_p2 CCTATTTCTTGCGCGTACCA CCCCAAGGCAAAAGTACGAT 50.0 50.0 58.4 58.2 492-555 bp 0.96 79.3
ycf4-cemA_p3 GCGCGTACCAATGGAAGTAT CCCCAAGGCAAAAGTACGAT 50.0 50.0 58.4 58.2 482-545 bp 0.96 78.7
ycf4-cemA_p4 ATTTCTTGCGCGTACCAATG CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.5 58.2 489-552 bp 0.96 77.8
ycf4-cemA_p5 AATTGGCCTATTTCTTGCGC CCCCAAGGCAAAAGTACGAT 45.0 50.0 57.4 58.2 498-561 bp 0.96 77.4