Hotspot

trnF-GAA-ndhJ

LSC · 48097-48673 · 577 bp

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Pi 0.00412
MarkerSeek score 58.10
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
577
variable_sites
29
pi
0.00412
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 517-731 bp 1.0 85.9
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 507-721 bp 1.0 85.4
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TCCACGCCTTAAACGTATCC 50.0 50.0 58.1 57.7 544-758 bp 1.0 84.5
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CCACGCCTTAAACGTATCCT 50.0 50.0 58.1 57.7 543-757 bp 1.0 84.4
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA ATCATCCACGCCTTAAACGT 50.0 45.0 58.1 57.6 548-762 bp 1.0 83.7