Hotspot

petN-trnC-GCA

LSC · 17794-18718 · 925 bp

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Pi 0.00741
MarkerSeek score 26.60
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
925
variable_sites
34
pi
0.00741
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.00
markerseek_score
26.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 974-1015 bp 1.0 77.1
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA CCAGTTCAAATCTGGGTGCC 50.0 55.0 58.2 59.1 976-1017 bp 1.0 76.7
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA CAGTTCAAATCTGGGTGCCG 50.0 55.0 58.2 59.5 975-1016 bp 1.0 73.8
petN-trnC-GCA_p4 AGCCCAAGCGAGACTTACTA AGGGGACTGCAAATCCTTTA 50.0 45.0 58.2 56.4 999-1040 bp 1.0 72.7
petN-trnC-GCA_p5 AGCCCAAGCGAGACTTACTA TGCAAATCCTTTATCCCCAGT 50.0 42.9 58.2 57.2 992-1033 bp 1.0 71.5