Hotspot

trnK-UUU-rps16

LSC · 3961-4518 · 558 bp

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Pi 0.00921
MarkerSeek score 37.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
558
variable_sites
25
pi
0.00921
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.00
markerseek_score
37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAACT 50.0 47.6 58.3 57.9 680-697 bp 1.0 75.9
trnK-UUU-rps16_p2 CATAGCCGCACTTAAAAGCC GGTGCTCAACCTACAAGAACT 50.0 47.6 57.5 57.9 685-702 bp 1.0 74.3
trnK-UUU-rps16_p3 CGCACTTAAAAGCCGAGTAC GGTGCTCAACCTACAAGAACT 50.0 47.6 57.2 57.9 679-696 bp 1.0 71.9
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAAC 50.0 50.0 58.3 56.4 680-697 bp 1.0 71.8
trnK-UUU-rps16_p5 TCTACCAAGAGTCTGGACGA GGTGCTCAACCTACAAGAACT 50.0 47.6 57.1 57.9 736-753 bp 1.0 70.9