Hotspot

rpoB

LSC · 65071-68289 · 3219 bp

Back to Stigmatodon

Pi 0.00155

MarkerSeek score 55.90

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3219
variable_sites: 5
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ACCAGGCGTTCGCTAATTAG	TAGCCGAACACGAAGGAAAG	50.0	50.0	58.1	57.9	295 bp	1.0	80.0
rpoB_p2	CCAGGCGTTCGCTAATTAGT	TAGCCGAACACGAAGGAAAG	50.0	50.0	58.1	57.9	294 bp	1.0	80.0
rpoB_p3	ACCAGGCGTTCGCTAATTAG	GGGGGTTTCCATTATAGCCG	50.0	55.0	58.1	58.1	309 bp	1.0	79.8
rpoB_p4	CCAGGCGTTCGCTAATTAGT	CGGGGGTTTCCATTATAGCC	50.0	55.0	58.1	58.1	309 bp	1.0	79.8
rpoB_p5	CCAGGCGTTCGCTAATTAGT	GGGGGTTTCCATTATAGCCG	50.0	55.0	58.1	58.1	308 bp	1.0	79.8