Hotspot

psbC

LSC · 97593-99014 · 1422 bp

Back to Stigmatodon

Pi 0.000703

MarkerSeek score 48.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	CCGAGCCAGAACAGAAAAGA	TTTCCCAAGAAATCCGTGCA	50.0	45.0	57.8	58.0	1582 bp	1.0	78.6
psbC_p2	CCGAGCCAGAACAGAAAAGA	GCAGCGGAAGATCCTGAATT	50.0	50.0	57.8	58.3	1565 bp	1.0	76.1
psbC_p3	CCGAGCCAGAACAGAAAAGA	AGGTATTCGTGCTTGGATGG	50.0	50.0	57.8	57.7	1509 bp	1.0	76.0
psbC_p4	CCGAGCCAGAACAGAAAAGA	GTTTCCCAAGAAATCCGTGC	50.0	50.0	57.8	57.7	1583 bp	1.0	75.9
psbC_p5	CCGAGCCAGAACAGAAAAGA	TTCCCAAGAAATCCGTGCAG	50.0	50.0	57.8	58.5	1581 bp	1.0	75.0