Hotspot

clpP

LSC · 112885-115038 · 2154 bp

Back to Stigmatodon

Pi 0.00325

MarkerSeek score 59.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2154
variable_sites: 7
pi: 0.00325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGTTGGTTTAGATCGATCT	ACCATTGATTGTTACGTTTCCA	42.9	36.4	56.4	56.5	2324-2350 bp	1.0	40.3
clpP_p2	TGGGTTGGTTTAGATCGATCTT	ACCATTGATTGTTACGTTTCCA	40.9	36.4	57.2	56.5	2324-2350 bp	1.0	40.3
clpP_p3	TGGGTTGGTTTAGATCGATCT	AACCATTGATTGTTACGTTTCCA	42.9	34.8	56.4	57.2	2325-2351 bp	1.0	40.3
clpP_p4	ATGGGTTGGTTTAGATCGATCT	ACCATTGATTGTTACGTTTCCA	40.9	36.4	57.0	56.5	2325-2351 bp	1.0	40.3
clpP_p5	TGGGTTGGTTTAGATCGATCT	ACCATTGATTGTTACGTTTCCAT	42.9	34.8	56.4	57.0	2324-2350 bp	1.0	40.3