Hotspot

trnW-CCA-trnP-UGG

LSC · 109391-109528 · 138 bp

Back to Stigmatodon

Pi 0

MarkerSeek score 50.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	203-205 bp	1.0	79.5
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	204-206 bp	1.0	79.5
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	192-194 bp	1.0	79.5
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	190-192 bp	1.0	79.5
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	193-195 bp	1.0	79.5