Hotspot

trnS-UGA-psbC

LSC · 97456-97592 · 137 bp

Back to Stigmatodon

Pi 0.0511

MarkerSeek score 67.10

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 7
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGTTGATAGCTCCGGTCTTG	CGTTTTTGGGATCTTCGTGC	55.0	50.0	58.1	58.0	568 bp	1.0	80.5
trnS-UGA-psbC_p2	GGTTGATAGCTCCGGTCTTG	GCGTTTTTGGGATCTTCGTG	55.0	50.0	58.1	58.0	569 bp	1.0	80.5
trnS-UGA-psbC_p3	GGTTGATAGCTCCGGTCTTG	TCTAAGGGGTCCCAATGGTT	55.0	50.0	58.1	57.9	533 bp	1.0	80.3
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	GCTCGACGTGCATTTGTATG	55.0	50.0	58.1	57.9	859 bp	1.0	79.8
trnS-UGA-psbC_p5	GGTTGATAGCTCCGGTCTTG	TCGACGTGCATTTGTATGGT	55.0	45.0	58.1	57.9	857 bp	1.0	79.7