Hotspot

ndhJ-trnF-GAA

LSC · 83998-84776 · 779 bp

Back to Stigmatodon

Pi 0.0013

MarkerSeek score 60.60

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	ATAGGCTGGCCCTTACGTAA	CCTCGTGTCACCAGTTCAAA	50.0	50.0	58.2	58.1	870-879 bp	1.0	78.9
ndhJ-trnF-GAA_p2	CCCGAAAGTTGGATAGGCTG	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.3	58.1	882-891 bp	1.0	77.8
ndhJ-trnF-GAA_p3	CCGAAAGTTGGATAGGCTGG	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.3	58.1	881-890 bp	1.0	77.8
ndhJ-trnF-GAA_p4	ATGCCCGAAAGTTGGATAGG	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.7	58.1	885-894 bp	1.0	77.7
ndhJ-trnF-GAA_p5	ATAGGCTGGCCCTTACGTAA	ATGGTCGGGATAGCTCAGTT	50.0	50.0	58.2	58.2	912-921 bp	1.0	77.6