Hotspot

trnV-UAC-ndhC

LSC · 81526-82321 · 796 bp

Back to Stigmatodon

Pi 0.00506

MarkerSeek score 62.30

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 4
pi: 0.00506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	CTAACCGGGGCTAAAACTCC	50.0	55.0	57.8	58.0	928-933 bp	1.0	78.9
trnV-UAC-ndhC_p2	GAGCCCAATGCAACAAACAA	CTAACCGGGGCTAAAACTCC	45.0	55.0	57.8	58.0	1015-1020 bp	1.0	78.5
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	CGGGGCTAAAACTCCTGAAA	50.0	50.0	57.8	57.8	923-928 bp	1.0	77.6
trnV-UAC-ndhC_p4	GAGCCCAATGCAACAAACAA	CGGGGCTAAAACTCCTGAAA	45.0	50.0	57.8	57.8	1010-1015 bp	1.0	77.3
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	TAACCGGGGCTAAAACTCCT	50.0	50.0	57.8	58.3	927-932 bp	1.0	76.5