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trnC-GCA-petN

LSC · 69715-70508 · 794 bp

Back to Stigmatodon

Pi 0.0063

MarkerSeek score 53.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 5
pi: 0.0063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	843-1028 bp	1.0	75.4
trnC-GCA-petN_p2	ATTTTGGATCGGATTTGGCG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	908-1093 bp	1.0	72.2
trnC-GCA-petN_p3	TTCAAATCTGGGTGTCGCC	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	841-1026 bp	1.0	70.7
trnC-GCA-petN_p4	TTTTGGATCGGATTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	59.2	58.2	907-1092 bp	1.0	69.7
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.5	57.1	842-1027 bp	1.0	69.5