Hotspot

trnI-GAU-rrn16S

IRb · 23056-23371 · 316 bp

Back to Stigmatodon

Pi 0.0443
MarkerSeek score 78.30
Primer available yes
Label trnI-GAU-rrn16S

Feature Metadata

label_name
trnI-GAU-rrn16S
feature_type
igs
region
IRb
length_bp
316
variable_sites
14
pi
0.0443
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16S_p1 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 410 bp 1.0 78.9
trnI-GAU-rrn16S_p2 CTATCCATGTGGCTGAGAGC CAAGGTAGCCGTACTGGAAG 55.0 55.0 57.8 57.7 442 bp 1.0 78.8
trnI-GAU-rrn16S_p3 CGCTGATGAGCACATTGAAC CAAGGTAGCCGTACTGGAAG 50.0 55.0 57.8 57.7 461 bp 1.0 78.7
trnI-GAU-rrn16S_p4 CTATCCATGTGGCTGAGAGC AGTGACTGGAGTGAAGTCGT 55.0 50.0 57.8 58.3 464 bp 1.0 78.6
trnI-GAU-rrn16S_p5 CGCTGATGAGCACATTGAAC AGTGACTGGAGTGAAGTCGT 50.0 50.0 57.8 58.3 483 bp 1.0 78.5