Hotspot

ycf1

SSC · 127787-133441 · 5655 bp

Back to Stewartia

Pi 0.00598

MarkerSeek score 46.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 157
pi: 0.00598
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCTCGCATAGAACCTTTGA	TGGGTTTCGACCAACAAACA	50.0	45.0	58.0	58.2	810-846 bp	1.0	84.7
ycf1_p2	GTCTTTGGGCTCGCATAGAA	TGGGTTTCGACCAACAAACA	50.0	45.0	58.0	58.2	817-853 bp	1.0	84.6
ycf1_p3	TCTTTGGGCTCGCATAGAAC	TGGGTTTCGACCAACAAACA	50.0	45.0	58.0	58.2	816-852 bp	1.0	84.6
ycf1_p4	TCTGCTATTTCTATCCGCGC	TGGGTTTCGACCAACAAACA	50.0	45.0	57.9	58.2	1073-1109 bp	1.0	83.9
ycf1_p5	AATCCGTTTCATGAGTCCGG	TGGGTTTCGACCAACAAACA	50.0	45.0	58.0	58.2	933-969 bp	0.952381	81.9