Hotspot

rpoA

LSC · 81188-82201 · 1014 bp

Back to Stewartia

Pi 0.00308

MarkerSeek score 37.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 15
pi: 0.00308
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TAATGGTTTCAGTACCCGCG	TTGTACGGGATGTAACCCCT	50.0	50.0	58.0	58.0	1530-1561 bp	1.0	83.1
rpoA_p2	TAATGGTTTCAGTACCCGCG	TAAGAGGTCGGGTGGTTTCT	50.0	50.0	58.0	58.0	1746-1777 bp	1.0	83.0
rpoA_p3	TAATGGTTTCAGTACCCGCG	CCGGTACTTGTGGATTCAGG	50.0	55.0	58.0	58.0	1716-1747 bp	1.0	83.0
rpoA_p4	TAATGGTTTCAGTACCCGCG	CGGTACTTGTGGATTCAGGG	50.0	55.0	58.0	58.0	1715-1746 bp	1.0	83.0
rpoA_p5	TAATGGTTTCAGTACCCGCG	AGGGGTACAAGAAGAGGGAC	50.0	55.0	58.0	58.0	1699-1730 bp	1.0	82.8