Hotspot

rpl16

LSC · 84478-85929 · 1452 bp

Back to Stewartia

Pi 0.005

MarkerSeek score 54.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1452
variable_sites: 36
pi: 0.005
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCCAAGACCTATTTCCTTTGGT	TCAAAACAAAAACGAGCTCT	45.5	35.0	58.0	54.0	1543-1594 bp	0.952381	42.0
rpl16_p2	CCCAAGACCTATTTCCTTTGGT	TCAAAACAAAAACGAGCTCTT	45.5	33.3	58.0	54.8	1543-1594 bp	0.952381	42.0
rpl16_p3	CCCAAGACCTATTTCCTTTGGT	TCAATCAAAACAAAAACGAGCTCT	45.5	33.3	58.0	58.1	1547-1598 bp	0.952381	41.9
rpl16_p4	CCCAAGACCTATTTCCTTTGGT	TCAATCAAAACAAAAACGAGCTC	45.5	34.8	58.0	56.8	1547-1598 bp	0.952381	41.9
rpl16_p5	CCCAAGACCTATTTCCTTTGGT	CAATCAAAACAAAAACGAGCTCT	45.5	34.8	58.0	56.8	1546-1597 bp	0.952381	41.9