Hotspot

rps15-ycf1

SSC · 127406-127786 · 381 bp

Back to Stewartia

Pi 0.0129

MarkerSeek score 41.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 381
variable_sites: 19
pi: 0.0129
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AACCCCTTCGTCTGTCTGTA	45.0	50.0	58.1	58.0	1348 bp	1.0	81.9
rps15-ycf1_p2	AACACCATGCATTGATGGGT	ACCCCTTCGTCTGTCTGTAA	45.0	50.0	58.1	58.0	1347 bp	1.0	81.9
rps15-ycf1_p3	CTTGTAGCTGGTGCAGTCAT	ACCCCTTCGTCTGTCTGTAA	50.0	50.0	57.9	58.0	1292 bp	1.0	81.7
rps15-ycf1_p4	TTCCCAATTGTTCTGGTGCA	AACCCCTTCGTCTGTCTGTA	45.0	50.0	57.9	58.0	1544 bp	1.0	81.5
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	ACCCCTTCGTCTGTCTGTAA	45.0	50.0	57.9	58.0	1543 bp	1.0	81.5