Hotspot

ndhF-rpl32

SSC · 116131-116988 · 858 bp

Back to Stewartia

Pi 0.00954

MarkerSeek score 54.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 858
variable_sites: 40
pi: 0.00954
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGCCCACATACGACGAA	AGCTTTTAAGGCTGCCCAAT	45.0	45.0	57.8	58.1	1049-1077 bp	1.0	83.8
ndhF-rpl32_p2	CAGGCCGTGTAAACCAAAAC	AGCTTTTAAGGCTGCCCAAT	50.0	45.0	57.9	58.1	1431-1459 bp	1.0	83.0
ndhF-rpl32_p3	CAGGCCGTGTAAACCAAAAC	AAAATCGGAATTCCCACCCC	50.0	50.0	57.9	58.1	1756-1803 bp	1.0	83.0
ndhF-rpl32_p4	CAGGCCGTGTAAACCAAAAC	GAATTTCCGGTCGAAAGGGA	50.0	50.0	57.9	57.9	1466-1494 bp	1.0	82.5
ndhF-rpl32_p5	CAGGCCGTGTAAACCAAAAC	TTCCGGTCGAAAGGGATTTC	50.0	50.0	57.9	57.9	1462-1490 bp	1.0	82.5