Hotspot

trnN-GUU-ndhF

IRb · 112172-113877 · 1706 bp

Back to Stewartia

Pi 0.00339

MarkerSeek score 59.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1706
variable_sites: 22
pi: 0.00339
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	CAAATATGTAGGGGGTGGGC	50.0	55.0	58.2	58.0	1525-2330 bp	1.0	84.3
trnN-GUU-ndhF_p2	AATCAGTTAACAGCCGACCG	CAAATATGTAGGGGGTGGGC	50.0	55.0	58.3	58.0	1506-2330 bp	1.0	83.3
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	CAAATATGTAGGGGGTGGGC	55.0	55.0	57.7	58.0	1533-2330 bp	1.0	82.9
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.2	57.8	1511-2316 bp	1.0	82.3
trnN-GUU-ndhF_p5	AATCAGTTAACAGCCGACCG	GTGGGCGCATCTCTTCTTAT	50.0	50.0	58.3	57.8	1492-2316 bp	1.0	81.3