Hotspot

petA-psbJ

LSC · 65904-66944 · 1041 bp

Back to Stewartia

Pi 0.00665

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 38
pi: 0.00665
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1143-1188 bp	1.0	82.2
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1144-1189 bp	1.0	82.2
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1110-1155 bp	1.0	82.0
petA-psbJ_p4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1111-1156 bp	1.0	79.6
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1138-1183 bp	1.0	79.4