Hotspot

accD-psaI

LSC · 61309-62037 · 729 bp

Back to Stewartia

Pi 0.0114

MarkerSeek score 64.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 38
pi: 0.0114
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCATGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	842-899 bp	1.0	70.4
accD-psaI_p2	TCCAGAAGGGCTTATTCGATC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.2	58.0	912-969 bp	1.0	69.7
accD-psaI_p3	CCAGAAGGGCTTATTCGATCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.2	58.0	911-968 bp	1.0	69.7
accD-psaI_p4	TTCCAGAAGGGCTTATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.2	58.0	913-970 bp	1.0	69.7
accD-psaI_p5	GCTCCATGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	848-905 bp	1.0	69.7