Hotspot

trnF-GAA-ndhJ

LSC · 51226-51933 · 708 bp

Back to Stewartia

Pi 0.00521

MarkerSeek score 53.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 20
pi: 0.00521
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGATAATCATCCGCGCTTGA	55.0	45.0	58.0	57.5	890-940 bp	1.0	77.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGATAATCATCCGCGCTTGA	50.0	45.0	58.1	57.5	854-904 bp	1.0	77.5
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGATAATCATCCGCGCTTGA	55.0	45.0	57.9	57.5	878-928 bp	1.0	76.8
trnF-GAA-ndhJ_p4	AAGAGGATGCATCGGGAATG	TGATAATCATCCGCGCTTGA	50.0	45.0	57.7	57.5	913-963 bp	1.0	75.7
trnF-GAA-ndhJ_p5	AATGAAGAGGATGCATCGGG	TGATAATCATCCGCGCTTGA	50.0	45.0	57.7	57.5	917-967 bp	1.0	75.7