Hotspot

psaA-ycf3

LSC · 44277-45012 · 736 bp

Back to Stewartia

Pi 0.0029

MarkerSeek score 55.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 10
pi: 0.0029
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	904-934 bp	1.0	81.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	906-936 bp	1.0	80.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	900-930 bp	1.0	79.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	902-932 bp	1.0	79.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	886-905 bp	0.952381	78.9