Hotspot

trnT-GGU-psbD

LSC · 33811-35326 · 1516 bp

Back to Stewartia

Pi 0.00652

MarkerSeek score 62.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1516
variable_sites: 56
pi: 0.00652
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1636-1663 bp	1.0	82.1
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1673-1700 bp	0.952381	80.8
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1663-1690 bp	0.952381	79.8
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1662-1689 bp	0.952381	78.6
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	AGAGCAATAAACCGGACCAG	50.0	50.0	57.9	57.6	1686-1713 bp	0.952381	78.5