Hotspot

psbM-trnD-GUC

LSC · 31399-32531 · 1133 bp

Back to Stewartia

Pi 0.00325

MarkerSeek score 55.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 22
pi: 0.00325
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1243-1262 bp	1.0	60.6
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1250-1269 bp	1.0	57.4
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1244-1263 bp	1.0	56.4
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	1249-1268 bp	1.0	54.9
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	1242-1261 bp	1.0	54.6