Hotspot

rps15-ycf1

SSC · 123701-124022 · 322 bp

Back to Stevenia

Pi 0.0321

MarkerSeek score 73.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 322
variable_sites: 10
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAATTCAACGGATCCCCTGC	ACACATGTATACGCGATTTCCA	50.0	40.9	58.5	58.2	410-419 bp	1.0	62.0
rps15-ycf1_p2	AAATTCAACGGATCCCCTGC	CACATGTATACGCGATTTCCA	50.0	42.9	58.5	56.5	409-418 bp	1.0	59.8
rps15-ycf1_p3	AAATTCAACGGATCCCCTGC	ACACATGTATACGCGATTTCC	50.0	42.9	58.5	56.5	410-419 bp	1.0	59.8
rps15-ycf1_p4	GGATCCCCTGCTTTCTTCTT	ACACATGTATACGCGATTTCCA	50.0	40.9	57.2	58.2	401-410 bp	1.0	59.7
rps15-ycf1_p5	CGGATCCCCTGCTTTCTTC	ACACATGTATACGCGATTTCCA	57.9	40.9	57.9	58.2	402-411 bp	1.0	57.5