Hotspot

trnL-CUA-ccsA

SSC · 114531-114644 · 114 bp

Back to Stevenia

Pi 0.0536

MarkerSeek score 71.80

Primer available yes

Label trnL-CUA-ccsA

Feature Metadata

label_name: trnL-CUA-ccsA
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 6
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CUA-ccsA_p1	GTTTTGAACAAGTTGCCGCT	TGCAGACTGAGGCATTTTGT	45.0	45.0	58.1	58.0	590-592 bp	1.0	81.3
trnL-CUA-ccsA_p2	AAGTTGCCGCTATGGTGAAA	TGCAGACTGAGGCATTTTGT	45.0	45.0	58.1	58.0	581-583 bp	1.0	81.2
trnL-CUA-ccsA_p3	TAGACACGCTGCTCTTAGGA	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.9	58.0	557-559 bp	1.0	81.0
trnL-CUA-ccsA_p4	CGAAGCATTTTGGCGAAGTT	TGCAGACTGAGGCATTTTGT	45.0	45.0	57.9	58.0	976 bp	0.5	59.5
trnL-CUA-ccsA_p5	CGAAGCATTTTGGCGAAGTT	TAACCACTGGGACTGGAGAG	45.0	55.0	57.9	58.1	1009 bp	0.5	59.1