Hotspot

rpl32-trnL-CUA

SSC · 113658-114450 · 793 bp

Back to Stevenia

Pi 0.0483
MarkerSeek score 79.30
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
793
variable_sites
38
pi
0.0483
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 TGGACATCGTTGAAAGCCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 916-920 bp 1.0 80.7
rpl32-trnL-CUA_p2 TTGGACATCGTTGAAAGCCT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 917-921 bp 1.0 80.7
rpl32-trnL-CUA_p3 TGGACATCGTTGAAAGCCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 909-913 bp 1.0 80.2
rpl32-trnL-CUA_p4 TTGGACATCGTTGAAAGCCT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 910-914 bp 1.0 80.2
rpl32-trnL-CUA_p5 TGGACATCGTTGAAAGCCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 914-918 bp 1.0 80.1