Hotspot

ndhF-rpl32

SSC · 112653-113498 · 846 bp

Back to Stevenia

Pi 0.0335

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 846
variable_sites: 28
pi: 0.0335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGTCCACATACGGCGAA	AACTTCGCCAAAATGCTTCG	45.0	45.0	57.8	57.9	1546 bp	0.5	58.2
ndhF-rpl32_p2	GCAATAGGTCGTGTGAACCA	AACTTCGCCAAAATGCTTCG	50.0	45.0	58.2	57.9	1932 bp	0.5	58.0
ndhF-rpl32_p3	TGCAATAGGTCGTGTGAACC	AACTTCGCCAAAATGCTTCG	50.0	45.0	58.2	57.9	1933 bp	0.5	58.0
ndhF-rpl32_p4	GGTCGTGTGAACCAAAAACC	AACTTCGCCAAAATGCTTCG	50.0	45.0	57.8	57.9	1926 bp	0.5	58.0
ndhF-rpl32_p5	AAAGTCCACATACGGCGAAG	AACTTCGCCAAAATGCTTCG	50.0	45.0	58.3	57.9	1545 bp	0.5	57.4