Hotspot

psbZ-trnG-GGC

LSC · 35715-36182 · 468 bp

Back to Stevenia

Pi 0.037
MarkerSeek score 66.00
Primer available yes
Label psbZ-trnG-GGC

Feature Metadata

label_name
psbZ-trnG-GGC
feature_type
igs
region
LSC
length_bp
468
variable_sites
17
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GGC_p1 TCCTGATGGTTGGTCGAGTA TACCATTCGACTATATCCGC 50.0 45.0 57.8 54.3 591-618 bp 1.0 51.7
psbZ-trnG-GGC_p2 TCCTGATGGTTGGTCGAGTA TTACCATTCGACTATATCCGC 50.0 42.9 57.8 55.1 592-619 bp 1.0 50.4
psbZ-trnG-GGC_p3 TTTGCGTCTCCTGATGGTTG TACCATTCGACTATATCCGC 50.0 45.0 58.5 54.3 599-626 bp 1.0 49.6
psbZ-trnG-GGC_p4 ATTTGCGTCTCCTGATGGTT TACCATTCGACTATATCCGC 45.0 45.0 57.5 54.3 600-627 bp 1.0 49.5
psbZ-trnG-GGC_p5 TCCTGATGGTTGGTCGAGTA TTTACCATTCGACTATATCCGC 50.0 40.9 57.8 55.9 593-620 bp 1.0 48.5