Hotspot

trnT-ACC-psbD

LSC · 31239-32469 · 1231 bp

Back to Stevenia

Pi 0.0252
MarkerSeek score 60.80
Primer available yes
Label trnT-ACC-psbD

Feature Metadata

label_name
trnT-ACC-psbD
feature_type
igs
region
LSC
length_bp
1231
variable_sites
31
pi
0.0252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACC-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1310-1323 bp 1.0 42.0
trnT-ACC-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1313-1326 bp 1.0 42.0
trnT-ACC-psbD_p3 CCAAGACGGCCCTTTTAACT TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1332-1345 bp 1.0 42.0
trnT-ACC-psbD_p4 ACGGCCCTTTTAACTCAGTG TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1327-1340 bp 1.0 42.0
trnT-ACC-psbD_p5 GACGGCCCTTTTAACTCAGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.8 53.0 1328-1341 bp 1.0 42.0