Hotspot

rpoC1-rpoB

LSC · 22965-22990 · 26 bp

Back to Stevenia

Pi 0.0769

MarkerSeek score 78.00

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 2
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TTTTAGTGGCCCAAGCACTT	ACTACGATCTTTGGCTCTGG	45.0	50.0	57.9	57.0	184 bp	1.0	72.5
rpoC1-rpoB_p2	TTTTAGTGGCCCAAGCACTT	ACGATCTTTGGCTCTGGAAT	45.0	45.0	57.9	56.9	181 bp	1.0	71.5
rpoC1-rpoB_p3	TTTTAGTGGCCCAAGCACTT	TCGAGAACTACGATCTTTGGC	45.0	47.6	57.9	57.9	190 bp	1.0	71.0
rpoC1-rpoB_p4	TTTTAGTGGCCCAAGCACTT	CGAGAACTACGATCTTTGGCT	45.0	47.6	57.9	57.8	189 bp	1.0	71.0
rpoC1-rpoB_p5	AGTGGCCCAAGCACTTATTT	ACTACGATCTTTGGCTCTGG	45.0	50.0	57.7	57.0	180 bp	1.0	70.7