Hotspot

trnG-GGA-trnR-AGA

LSC · 9284-9436 · 153 bp

Back to Stevenia

Pi 0.0533

MarkerSeek score 74.70

Primer available yes

Label trnG-GGA-trnR-AGA

Feature Metadata

label_name: trnG-GGA-trnR-AGA
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 8
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GGA-trnR-AGA_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	275-280 bp	1.0	50.3
trnG-GGA-trnR-AGA_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	281-286 bp	1.0	50.2
trnG-GGA-trnR-AGA_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	295-300 bp	1.0	49.9
trnG-GGA-trnR-AGA_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	283-288 bp	1.0	49.9
trnG-GGA-trnR-AGA_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	276-281 bp	1.0	49.3