Hotspot

trnS-AGC-trnG-GGA

LSC · 7813-8488 · 676 bp

Back to Stevenia

Pi 0.0402
MarkerSeek score 79.00
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
676
variable_sites
25
pi
0.0402
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA TGAATCAAACCGAGAGACCC 50.0 50.0 57.8 57.2 745-786 bp 1.0 79.2
trnS-AGC-trnG-GGA_p2 AACGCTTTAGTCCACTCAGC TGAATCAAACCGAGAGACCC 50.0 50.0 58.2 57.2 743-784 bp 1.0 78.9
trnS-AGC-trnG-GGA_p3 GCTTTAGTCCACTCAGCCAT TGAATCAAACCGAGAGACCC 50.0 50.0 57.6 57.2 740-781 bp 1.0 77.2
trnS-AGC-trnG-GGA_p4 TCCAACGCTTTAGTCCACTC TGAATCAAACCGAGAGACCC 50.0 50.0 57.5 57.2 746-787 bp 1.0 76.9
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA GAATCAAACCGAGAGACCCT 50.0 50.0 57.8 56.9 744-785 bp 1.0 76.8