Hotspot

ycf1

SSC · 126696-132155 · 5460 bp

Back to Sternbergia

Pi 0.0207

MarkerSeek score 52.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 110
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTGGTATGATCCACGGT	ACGCATATCGACACCAAGAT	45.0	45.0	56.3	57.1	124-253 bp	1.0	63.8
ycf1_p2	ACGCATCAAAAAGTAGCACA	ACGCATATCGACACCAAGAT	40.0	45.0	56.3	57.1	94-223 bp	1.0	63.6
ycf1_p3	TGATTGGTATGATCCACGGT	GGACCAATACGCATATCGACA	45.0	47.6	56.3	58.0	132-261 bp	1.0	62.7
ycf1_p4	TGATTGGTATGATCCACGGT	TGGACCAATACGCATATCGAC	45.0	47.6	56.3	58.0	133-262 bp	1.0	62.7
ycf1_p5	ACGCATCAAAAAGTAGCACA	GGACCAATACGCATATCGACA	40.0	47.6	56.3	58.0	102-231 bp	1.0	62.5