Hotspot

rpl22

LSC · 85475-85861 · 387 bp

Back to Sternbergia

Pi 0.0388

MarkerSeek score 76.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 15
pi: 0.0388
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	ATTCATTGGCGGGGGATAAC	47.6	50.0	58.1	58.0	495-502 bp	1.0	73.8
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	CATTCATTGGCGGGGGATAA	47.6	50.0	58.1	58.0	496-503 bp	1.0	73.8
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	TTATCATTCATTGGCGGGGG	47.6	50.0	58.1	58.0	500-507 bp	1.0	73.8
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	TCTGACTTTCGCGAGACATG	47.6	50.0	58.1	58.0	570-576 bp	1.0	73.5
rpl22_p5	CCAAGTCTGAAACCAAGTGGA	CCTACTCTGACTTTCGCGAG	47.6	55.0	58.1	57.8	575-581 bp	1.0	72.2