Hotspot

atpF

LSC · 12546-14127 · 1582 bp

Back to Sternbergia

Pi 0.0306

MarkerSeek score 65.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1582
variable_sites: 48
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCGAAGAGTTACCATTAGTGTCTC	AGCTCTGTTCTTTGTTAGTCCT	41.7	40.9	57.8	57.0	1716-1823 bp	1.0	42.8
atpF_p2	CGAAGAGTTACCATTAGTGTCTCT	AGCTCTGTTCTTTGTTAGTCCT	41.7	40.9	57.8	57.0	1715-1822 bp	1.0	42.8
atpF_p3	TCGAAGAGTTACCATTAGTGTCT	AGCTCTGTTCTTTGTTAGTCCT	39.1	40.9	56.8	57.0	1716-1823 bp	1.0	42.8
atpF_p4	TCGAAGAGTTACCATTAGTGTCTC	AGAGCTCTGTTCTTTGTTAGTCC	41.7	43.5	57.8	58.1	1718-1825 bp	1.0	42.8
atpF_p5	TCGAAGAGTTACCATTAGTGTCTC	GAGCTCTGTTCTTTGTTAGTCCT	41.7	43.5	57.8	58.1	1717-1824 bp	1.0	42.8