Hotspot

clpP-psbB

LSC · 74055-74489 · 435 bp

Back to Sternbergia

Pi 0.0115

MarkerSeek score 61.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 5
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CCCAACCAGAAACTAGAGCC	50.0	55.0	58.0	57.9	611 bp	1.0	79.7
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	613 bp	1.0	79.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	531 bp	1.0	78.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CCAACCAGAAACTAGAGCCG	50.0	55.0	58.0	58.3	610 bp	1.0	78.5
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	CCCAACCAGAAACTAGAGCC	50.0	55.0	57.8	57.9	557 bp	1.0	78.4