Hotspot

ndhJ-ndhK

LSC · 51101-51181 · 81 bp

Back to Sternbergia

Pi 0.0247

MarkerSeek score 61.20

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 2
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GCAATCGAAGCCCAAAGATC	CAAGGTTCTTTTGCGCAGAA	50.0	45.0	57.5	57.5	173 bp	1.0	73.1
ndhJ-ndhK_p2	GAGTCCCAATCCTCGGTTTT	CAAGGTTCTTTTGCGCAGAA	50.0	45.0	57.5	57.5	217 bp	1.0	72.9
ndhJ-ndhK_p3	GGCAATCGAAGCCCAAAGAT	CAAGGTTCTTTTGCGCAGAA	50.0	45.0	58.9	57.5	174 bp	1.0	69.8
ndhJ-ndhK_p4	AGTCCCAATCCTCGGTTTTT	CAAGGTTCTTTTGCGCAGAA	45.0	45.0	57.0	57.5	216 bp	1.0	69.0
ndhJ-ndhK_p5	GCAATCGAAGCCCAAAGATC	CAAGGTTCTTTTGCGCAGAAT	50.0	42.9	57.5	58.0	173 bp	1.0	68.9